📄 5-Amino-1MQ: Storage, Reconstitution & Stability Guide

Document ID: NV-GUIDE-5AMQ-01_v1.0
Effective: 03 Jan 2026 | NovaVitality — UK-Made RUO Peptides — Rigorously Verified, Responsibly Supplied

⚠️ Research Use Only (RUO) — For laboratory or educational purposes only. Not for human or veterinary use. By using this guide, you affirm compliance with institutional biosafety and chemical hygiene policies (COSHH recommended).

🔬 1. Product Overview

Parameter	Value
Product Name	5-Amino-1MQ Research Compound
Catalogue No.	NV-5AMQ-5
CAS Number	1373719-12-8 (assigned to 5-amino-1-methylquinolinium)
Chemical Name	1-Methyl-5-aminoquinolinium acetate
Molecular Formula	C₁₁H₁₁N₃ · CH₃COO⁻
Theoretical MW	243.70 g/mol (acetate salt)<br>186.24 g/mol ([M+H]⁺ for MS)
Observed MW (Typical CoA)	186.2 Da (LC-MS, [M+H]⁺)
Purity (Typical)	≥98% (HPLC, 254 nm, isocratic)
Form Supplied	Lyophilised solid (white to off-white, hygroscopic powder)

✅ All batches include CoA with HPLC, LC-MS, KF, acetic acid, residual solvents, endotoxin.

📦 2. Storage Recommendations

✅ Solid (Lyophilised) Form

Critical: 5-Amino-1MQ is highly hygroscopic — moisture uptake accelerates degradation.

Condition	Max Duration	Notes
–20°C or below, argon-flushed, desiccated	≥24 months	✅ Recommended. Store in sealed amber vial with ≥2 g silica desiccant. Minimise air headspace.
2–8°C, argon-flushed	≤3 months	Acceptable for active-use inventory. Monitor for clumping (sign of moisture).
Room Temp (15–25°C)	≤7 days	❌ Avoid — rapid water absorption → hydrolysis & purity loss.

📌 Never open vial at RT without pre-warming & desiccator use.

✅ Reconstituted (in Solution) Form

Assumes: anhydrous DMSO (≥99.9%) for stock; aqueous dilution for working solutions.

Solution Type	Condition	Max Duration	Notes
DMSO Stock (100 mM)	–80°C, aliquoted	6 months	✅ Recommended. Use low-bind polypropylene tubes. Avoid freeze–thaw >3×.
DMSO Stock (100 mM)	–20°C	1 month	Risk of DMSO crystallisation at <–40°C.
Aqueous Working (≤10 µM)	4°C	≤4 hours	Dilute from DMSO stock; final DMSO ≤0.1%. Use same-day.
Aqueous Working	RT	≤1 hour	Degrades rapidly — not recommended for quantitative assays.

⚠️ Critical Handling Tips:

✅ Always pre-warm vial to RT in desiccator before opening.

✅ Use anhydrous DMSO (store over 3Å molecular sieves).

✅ Prepare DMSO stock first, then dilute into assay buffer.

❌ Avoid water, humid air, or non-sealed containers.

💧 3. Step-by-Step Reconstitution Protocol

🧪 Recommended Solvent

Anhydrous DMSO (≥99.9%) — only for stock solution
Do NOT use water or saline directly on powder.

🔢 Dosage Example (5 mg vial)

Target Stock	DMSO Volume	Final Concentration
100 mM	0.254 mL	Standard for in vitro screening
10 mM	2.54 mL	For high-solubility assays

🛠️ Procedure

Equilibrate sealed vial in desiccator at RT for 30 min.
Sterilise rubber stopper with 70% ethanol.
Pierce with dry needle; inject DMSO slowly down vial wall.
Vortex 10 sec — solution clears rapidly (no sonication needed).
Aliquot (e.g., 25 µL) into low-bind tubes; store at –80°C.

📊 Tip: 5-Amino-1MQ DMSO stocks are clear and colourless. Turbidity = moisture contamination.

📈 4. Stability Verification (Post-Reconstitution)

Parameter	Method	Acceptance Criteria
Purity	HPLC (C18, 30% ACN/H₂O + 0.1% TFA, 254 nm)	≥95% of initial (e.g., ≥93% if CoA = 98%)
Identity	LC-MS (ESI+)	m/z 186.2 ± 0.3 ([M+H]⁺)
Bioactivity (Optional)	NNMT inhibition assay (recombinant hNNMT)	IC₅₀ ≤ 200 nM vs. reference

📅 For long-term studies: test DMSO stock at 0, 30, 90, 180 days.

⚠️ 5. Incompatibilities & Degradation Risks

Risk Factor	Effect	Mitigation
Moisture	Hydrolysis → quinoline ring cleavage	Use desiccator; argon-seal; KF ≤5% on CoA
Light (UV)	Photo-oxidation of aromatic ring	Store in amber vials; wrap in foil
Basic pH (>8)	Demethylation at N1	Keep stock in neutral DMSO; avoid Tris buffers
Metal ions (Fe³⁺)	Oxidation of amine group	Use ultrapure DMSO; add 0.1 mM EDTA if needed
Repeated freeze–thaw	DMSO crystallisation → concentration shift	Aliquot; ≤3 cycles

📎 6. Compliance & Documentation

✅ SDS Reference: NV-SDS-5AMQ-01_v1.0 (Non-hazardous RUO format)
✅ SOP Reference: NV-SOP-CHEM-001_v1: Hygroscopic Small Molecule Handling
✅ CoA Includes: HPLC, LC-MS, KF, acetic acid, residual solvents (ACN, DMSO), endotoxin
✅ Label: “RUO — Research Use Only” + QR → this guide

🔗 Full protocol downloads:
Reconstitution Guide

📝 7. Revision History

Version	Date	Changes	Approved By
v1.0	03 Jan 2026	Initial release	Ste, NovaVitality QA

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