📄 Ipamorelin: Storage, Reconstitution & Stability Guide

Document ID: NV-GUIDE-IPA-01_v1.0
Effective: 03 Jan 2026 | NovaVitality — UK-Made RUO Peptides — Rigorously Verified, Responsibly Supplied

⚠️ Research Use Only (RUO) — For laboratory or educational purposes only. Not for human or veterinary use. By using this guide, you affirm compliance with institutional biosafety and ethics policies.

🔬 1. Product Overview

Parameter	Value
Product Name	Ipamorelin Research Peptide
Catalogue No.	NV-IPA-5
CAS Number	170851-70-4
Sequence	H-Aib-His-D-2-Nal–D-Phe-Lys-NH₂<br>(Aib = α-aminoisobutyric acid; D-2-Nal = D-2-naphthylalanine)
Molecular Formula	C₃₈H₄₉N₉O₅
Theoretical MW	711.86 g/mol
Typical Observed MW (CoA)	711.8–712.0 Da (LC-MS, [M+H]⁺)
Purity (Typical)	≥98% (HPLC, 214 nm)
Form Supplied	Lyophilised solid (white to off-white powder)

✅ All batches include CoA with HPLC, LC-MS, KF, peptide content, endotoxin.

📦 2. Storage Recommendations

✅ Powder (Lyophilised) Form

Condition	Max Duration	Notes
–20°C or below	≥24 months	✅ Recommended. Store in sealed amber vial with desiccant, under argon if possible. Protect from light & moisture.
2–8°C (Refrigerated)	6–12 months	Acceptable for active-use inventory. Monitor for clumping (humidity ingress).
Room Temp (15–25°C)	≤30 days	Short-term handling only. Avoid >25°C — may accelerate D-amino acid racemisation over time.

📌 Never store in frost-free freezers (temperature cycling causes degradation).

✅ Reconstituted (in Solution) Form

Assumes: sterile bacteriostatic water (0.9% BA) or 0.9% NaCl, pH ~5–6, aliquoted in low-bind tubes.

Condition	Max Duration	Notes
–20°C	3–6 months	✅ Recommended for stock. Aliquot to avoid freeze–thaw. Stable due to Aib¹ & D-residues.
2–8°C	≤7 days	Ideal for multi-dose in vivo studies. No significant degradation observed at 4°C (Bowers, 2010).
Room Temp	≤24 hours	Use same-day for critical assays. Avoid agitation — may promote surface adsorption.

⚠️ Critical Handling Tips:

✅ No sonication or warming needed — dissolves rapidly (<60 sec) at RT.

✅ Use low-bind polypropylene tubes (e.g., Eppendorf LoBind) — minimises adsorption.

❌ Avoid nylon filters — use 0.22 µm PVDF for sterile filtration.

❌ Do not vortex vigorously — gentle swirl only.

💧 3. Step-by-Step Reconstitution Protocol

🧪 Recommended Solvent

Sterile bacteriostatic water (0.9% benzyl alcohol) — for in vivo rodent work
0.9% NaCl (sterile saline) — for in vitro assays
0.1% acetic acid — only if solubility issues arise (rare for Ipamorelin)

🔢 Dosage Example (5 mg vial)

Target Concentration	Solvent Volume	Notes
5 mg/mL	1.0 mL	Standard stock for in vivo dosing (e.g., 100 µg/kg)
1 mg/mL	5.0 mL	Working dilution for in vitro (e.g., 100 nM–10 µM)

🛠️ Procedure

Warm vial to RT (if stored at –20°C) — 5 min.
Sterilise rubber stopper with 70% ethanol.
Slowly add solvent down vial wall (avoid foaming).
Gently swirl — do not shake or vortex.
Let stand 30–60 sec — solution clears rapidly.
Aliquot into low-bind tubes; store per Section 2.

📊 Tip: Ipamorelin solutions are optically clear and colourless — turbidity indicates contamination or degradation.

📈 4. Stability Verification (Post-Reconstitution)

Parameter	Method	Acceptance Criteria
Purity	RP-HPLC (C18, 0.1% TFA/ACN, 214 nm)	≥95% of initial (e.g., ≥93% if CoA = 98%)
Identity	LC-MS (ESI+)	m/z 711.8–712.2 ([M+H]⁺)
Bioactivity (Optional)	GH ELISA (rat pituitary cells)	EC₅₀ ≤ 15 nM (vs. reference batch)

📅 For long-term studies: test at 0, 30, 60, 90 days.

⚠️ 5. Incompatibilities & Degradation Risks

Risk Factor	Effect	Mitigation
Repeated freeze–thaw	Minor aggregation	Aliquot; ≤3 cycles
High pH (>8.0)	D-2-Nal oxidation	Use pH 5–6 solvents
Light exposure	Aromatic residue degradation	Store in amber vials
Metal ions (Cu²⁺)	His² oxidation	Use ultrapure solvents
Polystyrene tubes	Peptide adsorption	Use polypropylene/low-bind

📎 6. Compliance & Documentation

✅ SDS Reference: NV-SDS-IPA-01_v1.0
✅ SOP Reference: NV-SOP-RECON-003_v1: Small Peptide Reconstitution
✅ CoA Includes: HPLC, LC-MS, KF, peptide content, endotoxin
✅ Label: “RUO — Research Use Only” + QR → this guide

🔗 Full protocol downloads:
Reconstitution Guide

📝 7. Revision History

Version	Date	Changes	Approved By
v1.0	03 Jan 2026	Initial release	Steven Simpson, NovaVitality QA

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