NAD+

NAD+ Research Compound

NAD⁺ (Nicotinamide Adenine Dinucleotide, oxidised form) is a endogenous pyridine nucleotide cofactor, engineered to investigate redox homeostasis, sirtuin activation, mitochondrial bioenergetics, and DNA repair signalling. It has become a pivotal compound in preclinical ageing, metabolism, and neurodegeneration research due to its central role in NAD⁺-consuming enzymes (e.g., SIRT1, PARP1, CD38) and its decline in age-related pathologies. This NAD⁺ research compound is supplied as a lyophilised solid to ensure stability, purity, and reproducibility in laboratory and investigative settings.

Supplied in lyophilised (freeze-dried) form to preserve stability.

What is the NAD⁺ Research Compound?

NAD⁺ (C₂₁H₂₇N₇O₁₄P₂, MW: 663.43 g/mol) is the oxidised form of nicotinamide adenine dinucleotide, composed of nicotinamide mononucleotide (NMN) and adenosine monophosphate (AMP) linked by a pyrophosphate bridge. Unlike commercial NAD⁺ supplements, NovaVitality’s NAD⁺ is:

• ≥98% pure (HPLC-UV, ion-pairing chromatography)
• Endotoxin-tested (<0.1 EU/mg) for in vitro cell work
• Validated for sirtuin deacetylase activity (SIRT1 fluorometric assay)
• Supplied exclusively for in vitro, ex vivo, and preclinical research — not for human or veterinary use.

🔬 Note: NAD⁺ is not licensed by the MHRA, FDA, or EMA for therapeutic use. Oral NAD⁺ has negligible bioavailability — this RUO material is intended solely for mechanistic cell biology and enzymology research.

Scientific Identifiers

Usage and Reconstitution

The NAD⁺ research compound is highly hygroscopic and pH-sensitive. For experimental protocols:
✅ Solvent: Ultrapure water (18.2 MΩ·cm) or assay buffer (pH 7.0–7.5)
❌ Avoid: DMSO, acetic acid, alkaline buffers (pH >8.0), or metal-contaminated water

⚠️ Critical Handling Notes:
– Dissolve immediately before use — NAD⁺ hydrolyses rapidly in solution (t₁/₂ ~4h at 25°C, pH 7.4).
– Do not vortex — shear forces accelerate degradation.
– Filter through 0.22 µm PVDF only if required (NAD⁺ is small — 663 Da).

Post-Reconstitution Storage:
– 2–8°C — ≤2 hours (for immediate assays)
– −80°C (aliquoted) — ≤1 week (≤1 freeze–thaw cycle)
– Never store at −20°C — accelerates hydrolysis vs. −80°C.

NAD⁺ Research Compound: Key Investigational Pathways

NAD⁺ is actively studied for its role in cellular energetics and signalling. Key research applications include:

• Sirtuin Activation & Deacetylation
  Investigated in:
  → Fluorometric SIRT1/3/6 activity assays (Fluor de Lys® substrates)
  → Acetyl-proteomics (LC-MS/MS) in neuronal cultures
  → PGC-1α deacetylation and mitochondrial biogenesis
• PARP & DNA Repair Dynamics
  Explored via:
  → PARylation assays (anti-PAR immunoblot) after H₂O₂/UV damage
  → Comet assay for DNA strand break resolution
  → NAD⁺ consumption kinetics (HPLC quantification)
• Mitochondrial Bioenergetics
  Studied in:
  → Seahorse XF Analyzer (OCR, ATP-linked respiration)
  → NAD⁺/NADH ratio imaging (Peredox biosensor)
  → Complex I activity (rotenone-sensitive NADH oxidation)
• Ageing & Senescence Modulation
  Evaluated in:
  → Senescence-associated β-galactosidase (SA-β-gal) in HDFs
  → p16/p21 expression after NAD⁺ repletion
  → CD38 inhibition synergy studies

⚠️ All accessories (syringes, filters, vials) are for laboratory use only — not medical devices.

Why Order NAD⁺ from NovaVitality?

• 🔬 Biochemical validation: Every lot includes a CoA
• 🇬🇧  UK-based & compliant: MHRA-aligned documentation, −80°C cold-chain handling, next-day tracked dispatch on dry ice
• 📚 Research-first ethos: No “anti-ageing” or “energy booster” claims — full support for enzymology and redox biology
• 🛡️ RUO integrity: Meets MHRA GMP Guide, FDA 21 CFR 1271, ISO 13485 expectations
• 💬 Expert support: Get help with NAD⁺/NADH ratio assays, sirtuin kinetics, or stability in media

Legal and Safety Information

NovaVitality supplies NAD⁺ strictly for laboratory research, assay development, and educational purposes (RUO).

❌ This product is not approved by the MHRA, FDA, EMA, or any regulatory authority for:
→ Human use
→ Veterinary use
→ Diagnostic, therapeutic, supplement, or cosmetic use

⚠️ Critical Responsibilities for Researchers:
– Use only under approved institutional biosafety protocols
– Comply with local ethics oversight
– Maintain full chain-of-custody documentation
– Do not compound, dispense, or administer to living subjects

🔒 NovaVitality disclaims all liability for unauthorised or off-label use. By purchasing, you affirm compliance with all applicable laws and institutional policies.

Ready to Advance Redox & Ageing Research?

📦 [Bacteriostatic Water]
🧪 [Research Syringes]
📄 [NAD⁺ Storage & Stability Datasheet]