⚠️ Legal Notice
This product is supplied strictly for laboratory research or educational use by qualified personnel. It is not for human or veterinary use, and must not be administered to living organisms. By ordering, you affirm you are 18+ and a trained researcher, and will use this material solely for in vitro or ex vivo studies.

About CJC-1295 With DAC (RUO)

CJC-1295 With DAC (Drug Affinity Complex) 2mg is a modified growth hormone-releasing hormone (GHRH) analog used in laboratory research to study receptor binding kinetics and signal duration. The addition of DAC (a lysine-derivatised albumin-binding moiety) extends its half-life in biological matrices, making it useful for experiments requiring sustained exposure.

Common Research Contexts

🔬 Studying GHRH receptor activation in pituitary cell lines (e.g., AtT-20)
🔬 Comparing signal duration between DAC and non-DAC analogs
🔬 Supporting tissue explant studies where prolonged peptide stability is needed
🔬 Use as a reference compound in receptor-binding assays

RUO — Research Use Only

Always confirm suitability for your specific model system. Results may vary by tissue type and experimental design.

Typical Lab Use

Parameter	Typical Range	Notes
Working concentration	10 – 100 nM	≈ 5.9 – 59 µg/mL (MW = 3,368.2 g/mol)
Models studied	Pituitary cells (AtT-20), hypothalamic explants, serum stability assays
Exposure time	4 – 72 hours	DAC extends stability in serum-containing media
Solubility	≥5 mg/mL in 0.1% acetic acid	May precipitate in neutral PBS

Example Lab Workflow

Culture AtT-20 murine pituitary cells to 80% confluency
Starve 4 h in serum-free DMEM
Add 50 nM CJC-1295 With DAC in assay buffer
Incubate 24 h; collect supernatant for GH ELISA

📦 Product Information

Form	White to off-white lyophilised powder
Purity	≥98% (HPLC-UV)
Sequence	Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH₂ + DAC
MW	3,368.2 g/mol (TFA salt)
Endotoxin	<1.0 EU/mg
CoA	Yes — batch-specific (HPLC, MS, AA analysis)

🧊 Storage Guidance

Form	Storage	Stability
Powder (unopened)	–20°C, dry, dark	2+ years
Reconstituted stock	–80°C, single-use aliquots	6+ months
Working solution	4°C, in acidic buffer (pH 4.0)	Up to 48 h

🧴 Handling Tips

Equilibrate vial to room temperature before opening (15–20 min).
Use 0.1% acetic acid in sterile water for reconstitution.
Typical stock: 1–5 mg/mL — vortex gently, avoid foaming.
Aliquot and freeze at –80°C to prevent degradation.
Always label with “RUO — For Research Use Only”.

✅ Regulatory Notes
• Complies with UK REACH as a downstream user
• Not a medicinal product — for lab research only
• Safe handling: wear gloves, lab coat, and eye protection
• Dispose of peptide waste via licensed biological carrier

📚 Key References

J Clin Endocrinol Metab (2006)— Teichman SL et al. Prolonged stimulation of GH and IGF-1 by CJC-1295 in healthy adults

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J Clin Endocrinol Metab (2006) — Ionescu M et al. Pulsatile GH secretion persists during continuous CJC-1295 stimulation

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Am J Physiol Endocrinol Metab (2006) — Alba M et al. Once-daily CJC-1295 normalizes growth in GHRH knockout mouse

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StatPearls (NCBI Bookshelf) — Brinkman JE et al. Physiology, Growth Hormone

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CDC Pink Book (Chapter 6) — Vaccine administration: subcutaneous route (angle/site; no aspiration)

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Johns Hopkins Arthritis Center — How to give a subcutaneous injection (patient guidance)

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StatPearls (NCBI Bookshelf) — Regular Insulin: subcutaneous administration and site rotation

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Immunize Canada — How to Vaccinate: Best Practices (subcutaneous injection technique)

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Postgrad Med J (2006) — Ayuk J, Sheppard MC. Growth hormone and its disorders

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