CJC-1295 No DAC Research Peptide

CJC-1295 No DAC Research Peptide

CJC-1295 No DAC (Modified GRF 1–29) is a synthetic, 29-amino-acid analogue of growth hormone-releasing hormone (GHRH), engineered to investigate pulsatile GH secretion, somatotrope sensitivity, and GHRH receptor (GHRHR) signalling dynamics. It has become a pivotal compound in preclinical endocrinology, muscle metabolism, and receptor pharmacology research due to its enhanced stability over native GRF and clean pharmacokinetic profile (no albumin-binding DAC moiety). This CJC-1295 No DAC research peptide is supplied as a lyophilised solid to ensure structural integrity, purity, and reproducibility in laboratory and investigative settings.

Supplied in lyophilised (freeze-dried) form to preserve stability.

What is the CJC-1295 No DAC Research Peptide?

CJC-1295 No DAC (C₁₅₂H₂₅₄N₄₄O₄₂, MW: 3367.95 g/mol) is a modified GRF(1–29) analogue with four key substitutions (Ala² → D-Ala², Asp⁸ → Glu⁸, Ala¹⁵ → Leu¹⁵, Gly²⁷ → Ala²⁷) and C-terminal amidation, conferring resistance to dipeptidyl peptidase-4 (DPP-4) degradation [Veldhuis JD et al., J Clin Endocrinol Metab 2007; 92(3):957–964; DOI: 10.1210/jc.2006-2115]. Unlike DAC-containing variants, this No DAC form lacks the lysine-based drug affinity complex (DAC), resulting in a shorter half-life (~30 min in rodents) ideal for pulsatility studies. Supplied exclusively for in vitro, ex vivo, and preclinical research — not for human or veterinary use.

🔬 Note: CJC-1295 No DAC is distinct from CJC-1295 with DAC (CAS 876168-10-2). This RUO material is unformulated, preservative-free, and intended solely for GHRH receptor pharmacology and GH pulse dynamics research.

Scientific Identifiers

Usage and Reconstitution

The CJC-1295 No DAC research peptide is supplied freeze-dried for stability. For experimental protocols, reconstitute using:
✅ Sterile bacteriostatic water (0.9% benzyl alcohol in 0.9% NaCl), or
✅ 0.1% acetic acid in sterile saline — recommended for solubility (hydrophobic residues).

⚠️ Critical Handling Notes:
– Gentle warming (≤37°C) and brief sonication may aid dissolution.
– Avoid vigorous vortexing — may promote aggregation of hydrophobic core (Phe⁶, Leu¹⁵, Leu²²).
– Filter through 0.22 µm PVDF for cell-based assays.

Post-Reconstitution Storage:
– Short-term: 2–8°C for ≤72 hours
– Long-term: Aliquot and store at −80°C (≤3 freeze–thaw cycles)

🛒 Recommended reconstitution accessories will be auto-added to cart → Adjust before checkout.

CJC-1295 No DAC Research Peptide: Key Investigational Pathways

CJC-1295 No DAC is actively studied for its clean GHRH receptor activation. Key research applications include:

• GHRH Receptor Pharmacology
  Investigated in:
  → HEK293 cells stably expressing hGHRHR for cAMP, IP₁, and β-arrestin bias profiling
  → Competitive binding vs. native GRF(1–44) and Tesamorelin
  → Receptor internalisation kinetics (SNAP-tag confocal imaging)
• Pulsatile GH Secretion Dynamics
  Explored via:
  → Primary rat pituitary cell cultures (GH ELISA, 10-min sampling for pulse resolution)
  → Hypophyseal portal blood sampling in conscious rodents (GH pulse frequency/amplitude)
  → Somatostatin receptor (SSTR) co-modulation studies
• Metabolic & Anabolic Signalling
  Studied in:
  → C2C12 myotubes (IGF-1 secretion, Akt/mTOR phosphorylation)
  → Primary hepatocytes (IGF-1, ALS, IGFBP-3 expression)
  → DIO murine models (lean mass, glucose tolerance, insulin sensitivity)
• Receptor Desensitisation & Re-sensitisation
  Evaluated in:
  → Chronic vs. pulsatile dosing regimens (GH pulse recovery kinetics)
  → GRK2/5 recruitment (NanoBiT)
  → GHRHR re-sensitisation time-course after washout

⚠️ All accessories (syringes, filters, vials) are for laboratory use only — not medical devices.

Why Order CJC-1295 No DAC from NovaVitality?

• 🔬 Structural validation: Every lot includes a CoA
• 🇬🇧  UK-based & compliant: MHRA-aligned documentation, GMP-adjacent handling, next-day tracked dispatch
• 📚 Research-first ethos: No “HGH booster” or performance claims — full support for receptor pharmacology
• 🛡️ RUO integrity: Meets MHRA GMP Guide, FDA 21 CFR 1271, EU Annex 1 expectations
• 💬 Expert support: Get help with pulse dosing protocols, cAMP/β-arrestin assay design, or pituitary cell culture

Legal and Safety Information

NovaVitality supplies CJC-1295 No DAC strictly for laboratory research, assay development, and educational purposes (RUO).

❌ This product is not approved by the MHRA, FDA, EMA, or any regulatory authority for:
→ Human use
→ Veterinary use
→ Diagnostic, therapeutic, or performance-enhancement use

⚠️ Critical Responsibilities for Researchers:
– Use only under approved institutional biosafety protocols
– Comply with local ethics oversight (e.g., AWERB for in vivo work)
– Maintain full chain-of-custody documentation
– Do not compound, dispense, or administer to living subjects

🔒 NovaVitality disclaims all liability for unauthorised or off-label use. By purchasing, you affirm compliance with all applicable laws and institutional policies.

Ready to Advance GHRH Receptor & GH Pulse Research?

📦 [Bacteriostatic Water]
💉 [Research Syringes]
📄 [CJC-1295 No DAC Storage & Stability Datasheet]