Supplied for research purposes only. These peptides are laboratory reagents intended for qualified researchers and educational institutions. Not for human use, animal use, or clinical application.
NAD⁺ (Nicotinamide Adenine Dinucleotide, oxidised form) is a endogenous pyridine nucleotide cofactor, engineered to investigate redox homeostasis, sirtuin activation, mitochondrial bioenergetics, and DNA repair signalling. It has become a pivotal compound in preclinical ageing, metabolism, and neurodegeneration research due to its central role in NAD⁺-consuming enzymes (e.g., SIRT1, PARP1, CD38) and its decline in age-related pathologies. This NAD⁺ research compound is supplied as a lyophilised solid to ensure stability, purity, and reproducibility in laboratory and investigative settings.
Supplied in lyophilised (freeze-dried) form to preserve stability.
What is the NAD⁺ Research Compound?
NAD⁺ (C₂₁H₂₇N₇O₁₄P₂, MW: 663.43 g/mol) is the oxidised form of nicotinamide adenine dinucleotide, composed of nicotinamide mononucleotide (NMN) and adenosine monophosphate (AMP) linked by a pyrophosphate bridge. Unlike commercial NAD⁺ supplements, NovaVitality’s NAD⁺ is:
≥98% pure (HPLC-UV, ion-pairing chromatography)
Endotoxin-tested (<0.1 EU/mg) for in vitro cell work
Validated for sirtuin deacetylase activity (SIRT1 fluorometric assay)
Supplied exclusively for in vitro, ex vivo, and preclinical research — not for human or veterinary use.
🔬 Note: NAD⁺ is not licensed by the MHRA, FDA, or EMA for therapeutic use. Oral NAD⁺ has negligible bioavailability — this RUO material is intended solely for mechanistic cell biology and enzymology research.
Scientific Identifiers
Field
Value
Product Name
NAD⁺ (Nicotinamide Adenine Dinucleotide, Oxidised) Research Compound
Catalogue Numbers
NV-NAD-500 / NV-NAD-1000
CAS Number
53-84-9
Molecular Formula
C₂₁H₂₇N₇O₁₄P₂
Molecular Weight
663.43 g/mol (monoisotopic)
Chemical Structure
β-Nicotinamide–ribose–P–P–ribose–adenine
Form
Lyophilised Solid (white to off-white powder)
Purity
≥98% (HPLC-UV, ion-pairing C18, 260 nm)
Endotoxin
<0.1 EU/mg (LAL kinetic assay — critical for cell assays)
Storage
Store at −80°C, desiccated, protected from light and moisture
Unit Sizes
500 mg, 1000 mg
Usage and Reconstitution
The NAD⁺ research compound is highly hygroscopic and pH-sensitive. For experimental protocols: ✅ Solvent: Ultrapure water (18.2 MΩ·cm) or assay buffer (pH 7.0–7.5) ❌ Avoid: DMSO, acetic acid, alkaline buffers (pH >8.0), or metal-contaminated water
⚠️ Critical Handling Notes: – Dissolve immediately before use — NAD⁺ hydrolyses rapidly in solution (t₁/₂ ~4h at 25°C, pH 7.4). – Do not vortex — shear forces accelerate degradation. – Filter through 0.22 µm PVDF only if required (NAD⁺ is small — 663 Da).
Post-Reconstitution Storage: – 2–8°C — ≤2 hours (for immediate assays) – −80°C (aliquoted) — ≤1 week (≤1 freeze–thaw cycle) – Never store at −20°C — accelerates hydrolysis vs. −80°C.
NAD⁺ Research Compound: Key Investigational Pathways
NAD⁺ is actively studied for its role in cellular energetics and signalling. Key research applications include:
Sirtuin Activation & Deacetylation
Investigated in:
→ Fluorometric SIRT1/3/6 activity assays (Fluor de Lys® substrates)
→ Acetyl-proteomics (LC-MS/MS) in neuronal cultures
→ PGC-1α deacetylation and mitochondrial biogenesis
PARP & DNA Repair Dynamics
Explored via:
→ PARylation assays (anti-PAR immunoblot) after H₂O₂/UV damage
→ Comet assay for DNA strand break resolution
→ NAD⁺ consumption kinetics (HPLC quantification)
📚 Research-first ethos: No “anti-ageing” or “energy booster” claims — full support for enzymology and redox biology
🛡️ RUO integrity: Meets MHRA GMP Guide, FDA 21 CFR 1271, ISO 13485 expectations
💬 Expert support: Get help with NAD⁺/NADH ratio assays, sirtuin kinetics, or stability in media
Legal and Safety Information
NovaVitality supplies NAD⁺ strictly for laboratory research, assay development, and educational purposes (RUO).
❌ This product is not approved by the MHRA, FDA, EMA, or any regulatory authority for: → Human use → Veterinary use → Diagnostic, therapeutic, supplement, or cosmetic use
⚠️ Critical Responsibilities for Researchers: – Use only under approved institutional biosafety protocols – Comply with local ethics oversight – Maintain full chain-of-custody documentation – Do not compound, dispense, or administer to living subjects
🔒 NovaVitality disclaims all liability for unauthorised or off-label use. By purchasing, you affirm compliance with all applicable laws and institutional policies.
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