TB-500 (Thymosin Beta-4)

TB-500 (Thymosin Beta-4) Research Peptide

TB-500 is a synthetic, 43-amino-acid fragment of Thymosin β4, engineered to investigate G-actin sequestration, cell migration, and tissue repair signalling pathways. It has become a pivotal compound in preclinical wound healing, musculoskeletal regeneration, and anti-fibrotic research due to its potent effects on cytoskeletal dynamics, angiogenesis, and inflammatory modulation. This TB-500 research peptide is supplied as a lyophilised solid to ensure structural integrity, purity, and reproducibility in laboratory and investigative settings.

Supplied in lyophilised (freeze-dried) form to preserve stability.

What is the TB-500 (Thymosin Beta-4) Research Peptide?

TB-500 (C₂₁₂H₃₄₆N₆₂O₆₆S₃, MW: 4963.37 g/mol) is the N-terminal 1–43 fragment of full-length Thymosin β4 (residues 1–43), with the sequence Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Ser-OH, preserving the critical LKKTET actin-binding domain and N-terminal acetylation for stability [Bock SC et al., J Biol Chem 1989; 264(8):4644–4649; DOI: 10.1016/S0021-9258(18)81679-8]. Unlike uncharacterised commercial variants, NovaVitality’s TB-500 is synthesised with Met⁶ intact and validated by LC-MS and pyrene-actin polymerisation assays. Supplied exclusively for in vitro, ex vivo, and preclinical research — not for human or veterinary use.

🔬 Note: TB-500 is not licensed by the MHRA, FDA, or EMA. It is supplied under strict RUO (Research Use Only) protocols. Full-length Thymosin β4 (CAS 77530-35-3) is distinct from this fragment.

Scientific Identifiers

Usage and Reconstitution

The TB-500 research peptide is supplied freeze-dried for stability. For experimental protocols, reconstitute using:
✅ Sterile bacteriostatic water (0.9% benzyl alcohol in 0.9% NaCl), or
✅ 0.1% acetic acid in sterile saline — recommended for solubility (Lys-rich domain).

⚠️ Critical Handling Notes:
– Gentle warming (≤37°C) and brief sonication may aid dissolution.
– Avoid neutral/basic buffers (pH >7.0) — promotes aggregation due to Lys charge neutralisation.
– Filter through 0.22 µm PVDF for cell-based assays.

Post-Reconstitution Storage:
– Short-term: 2–8°C for ≤72 hours
– Long-term: Aliquot and store at −80°C (≤3 freeze–thaw cycles)

🛒 Recommended reconstitution accessories will be auto-added to cart → Adjust before checkout.

TB-500 Research Peptide: Key Investigational Pathways

TB-500 is actively studied for its cytoskeletal and regenerative bioactivity. Key research applications include:

• Actin Dynamics & Cell Migration
  Investigated in:
  → Pyrene-actin polymerisation assays (G-actin sequestration, critical concentration shift)
  → Fibroblast/keratinocyte scratch assays (migration speed, directionality)
  → Live-cell TIRF microscopy (F-actin turnover, focal adhesion disassembly)
• Wound Healing & Angiogenesis
  Explored via:
  → Diabetic murine excisional wounds (closure rate, CD31⁺ vessel density)
  → HUVEC tube formation (Matrigel, branch point quantification)
  → 3D skin equivalents (re-epithelialisation, tensile strength)
• Anti-Fibrotic & Anti-Inflammatory Effects
  Studied in:
  → Keloid fibroblasts (α-SMA, collagen contraction, MMP-1/TIMP-1)
  → LPS-stimulated macrophages (M1→M2 polarisation, IL-10/TNF-α ratio)
  → Post-surgical adhesion models (peritoneal, tendon sheath histology)
• Musculoskeletal Repair
  Evaluated in:
  → Rat Achilles tendon rupture (load-to-failure, collagen alignment, qPCR for scleraxis)
  → Equine superficial digital flexor tendon (SDFT) explants
  → Myoblast fusion assays (myotube formation, MyoD expression)

⚠️ All accessories (syringes, filters, vials) are for laboratory use only — not medical devices.

Why Order TB-500 from NovaVitality?

• 🔬 Structural validation: Every lot includes a CoA 
• 🇬🇧  UK-based & compliant: MHRA-aligned documentation, GMP-adjacent handling, next-day tracked dispatch
• 📚 Research-first ethos: No “regeneration” or “healing” claims — full support for mechanistic cytoskeletal studies
• 🛡️ RUO integrity: Meets MHRA GMP Guide, FDA 21 CFR 1271, EU Annex 1 expectations
• 💬 Expert support: Get help with actin assay setup, dosing in tendon models, or stability in culture media

Legal and Safety Information

NovaVitality supplies TB-500 strictly for laboratory research, assay development, and educational purposes (RUO).

❌ This product is not approved by the MHRA, FDA, EMA, or any regulatory authority for:
→ Human use
→ Veterinary use
→ Diagnostic, therapeutic, or cosmetic use

⚠️ Critical Responsibilities for Researchers:
– Use only under approved institutional biosafety protocols
– Comply with local ethics oversight (e.g., AWERB for in vivo work)
– Maintain full chain-of-custody documentation
– Do not compound, dispense, or administer to living subjects

🔒 NovaVitality disclaims all liability for unauthorised or off-label use. By purchasing, you affirm compliance with all applicable laws and institutional policies.

Ready to Advance Cytoskeletal & Tissue Repair Research?

📦 [Bacteriostatic Water]
💉 [Research Syringes]
📄 [TB-500 Storage & Stability Datasheet]