MOTS-c Research Peptide

MOTS-c – High-Purity Research Peptide

MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c) is a mitochondrial-derived peptide (MDP) encoded within the mitochondrial 12S rRNA gene, engineered to investigate metabolic homeostasis, insulin sensitisation, and AMPK-mediated stress adaptation. It has emerged as a pivotal compound in preclinical ageing, obesity, and exercise-mimetic research due to its unique nuclear translocation and folate-cycle modulation. This MOTS-c research peptide is supplied as a lyophilised solid to ensure stability, purity, and reproducibility in laboratory and investigative settings.

Supplied in lyophilised (freeze-dried) form to preserve stability.

What is the MOTS-c Research Peptide?

MOTS-c (C₅₈H₉₁N₁₇O₁₇S₁, MW: 1343.52 g/mol) is a 16-amino-acid peptide with the sequence Met-Arg-Trp-Glu-Glu-Glu-Glu-Glu-Glu-Glu-Glu-Glu-Glu-Glu-Glu-Glu, derived from the MT-RNR1 gene (human mitochondrial DNA, nt 680–727). Unlike uncharacterised analogues, NovaVitality’s MOTS-c is synthesised with N-terminal methionine intact (critical for bioactivity) and validated by LC-MS and functional AMPK phosphorylation assays [Lee C et al., Cell Metab 2015; 22(5):801–809; DOI: 10.1016/j.cmet.2015.09.025]. Supplied exclusively for in vitro, ex vivo, and preclinical research — not for human or veterinary use.

🔬 Note: MOTS-c is not licensed by the MHRA, FDA, or EMA. It is supplied under strict RUO (Research Use Only) protocols.

Scientific Identifiers

Usage and Reconstitution

The MOTS-c research peptide is supplied freeze-dried for stability. For experimental protocols, reconstitute using:
✅ Sterile bacteriostatic water (0.9% benzyl alcohol in 0.9% NaCl), or
✅ 0.1% acetic acid in sterile saline — recommended for solubility (high Glu content).

⚠️ Critical Handling Notes:
– Gentle warming (≤37°C) and brief sonication may aid dissolution.
– Avoid neutral/basic buffers (pH >7.0) — promotes aggregation due to Glu-rich domain.
– Filter through 0.22 µm PVDF for cell-based assays.

Post-Reconstitution Storage:
– Short-term: 2–8°C for ≤72 hours
– Long-term: Aliquot and store at −80°C (≤3 freeze–thaw cycles)

🛒 Recommended reconstitution accessories will be auto-added to cart → Adjust before checkout.

MOTS-c Research Peptide: Key Investigational Pathways

MOTS-c is actively studied for its nuclear-mitochondrial crosstalk and metabolic reprogramming. Key research applications include:

• AMPK Activation & Metabolic Sensing
  Investigated in:
  → Skeletal myotubes for p-AMPK (Thr172), GLUT4 translocation, fatty acid oxidation
  → HepG2 cells for suppression of de novo lipogenesis (ACC, FASN)
  → AICAR-synergy studies
• Folate Cycle & Redox Regulation
  Explored via:
  → MTHFD1L inhibition and AICAR accumulation
  → NAD⁺/NADH ratio modulation (LC-MS metabolomics)
  → Protection against H₂O₂-induced senescence
• Ageing & Stress Resistance
  Studied in:
  → C. elegans lifespan assays (require Met¹ intact)
  → Primary human fibroblasts for SA-β-gal, p16, mitochondrial membrane potential
  → Exercise-mimetic gene signatures (PGC-1α, NRF1)
• Obesity & Insulin Sensitisation
  Evaluated in:
  → DIO murine models for glucose tolerance (IPGTT), insulin signalling (p-AKT)
  → Adipose tissue macrophage polarisation (CD206⁺ M2 shift)
  → Browning of white adipocytes (UCP1, CIDEA)

⚠️ All accessories (syringes, filters, vials) are for laboratory use only — not medical devices.

Why Order MOTS-c from NovaVitality?

• 🔬 Met¹-intact validation: Every lot includes a CoA
• 🇬🇧  UK-based & compliant: MHRA-aligned documentation, GMP-adjacent handling, next-day tracked dispatch
• 📚 Research-first ethos: No anti-ageing or supplement claims — full support for mechanistic studies
• 🛡️ RUO integrity: Meets MHRA GMP Guide, FDA 21 CFR 1271, EU Annex 1
• 💬 Expert support: Get help with *dosing in C. elegans, stability in culture media, or nuclear localisation assays

Legal and Safety Information

NovaVitality supplies MOTS-c strictly for laboratory research, assay development, and educational purposes (RUO).

❌ This product is not approved by the MHRA, FDA, EMA, or any regulatory authority for:
→ Human use
→ Veterinary use
→ Diagnostic use
→ Therapeutic or supplement use

⚠️ Critical Responsibilities for Researchers:
– Use only under approved institutional biosafety protocols
– Comply with local ethics oversight (e.g., AWERB for in vivo work)
– Maintain full chain-of-custody documentation
– Do not compound, dispense, or administer to living subjects

🔒 NovaVitality disclaims all liability for unauthorised or off-label use. By purchasing, you affirm compliance with all applicable laws and institutional policies.

Ready to Advance Mitochondrial Signalling Research?

📦 [Bacteriostatic Water]
💉 [Research Syringes]
📄 [MOTS-c Storage & Stability Datasheet]